cis-2-amino-1-hydroxycyclobutane-1-acetic acid, a herbicidal antimetabolite produced by Streptomyces rochei A13018.

temperature was 27°C. On International Streptomyces Project (ISP) media 1, 2, 3, and 5, the strain produced yellow diffusible pigment and the reverse side color of the colonies was yellow. Melanin production was not observed on either tyrosine or peptone iron agar. The following tests were positive: starch hydrolysis; degradation of allantoin, lecithin, pectin, xanthine, and hypoxanthine; resistance to rifampicin (50//g/ml); tolerance to sodium azide (0.01 % w/v); utilization of D-glucose, D-raffinose, a-lactose, L-arabinose, D-galactose, L-valine, salicin, a-( + )-melibiose, D-fructose, D-mannitol, ( + )-mannose, L-sorbose, D-ribose, (+)-rhamnose, (+)maltose, sucrose, (+)-xylose, rayo-inositol, and